Fig. 1. Temporal patterns of vaccine-elicited T cell and Ab responses.

(A) and (B) IFN-γ producing CD4 ICS responses to Gag and Env; (C) and (D) IFN-γ producing CD8 ICS responses to Gag and Env; (E) and (F) Env-specific IgG in serum; (G) and (H) Gag,Pol plus Env-specific IgA in rectal secretions. Responses elicited by the DDMM regimen (turquoise) are in the left column; and those elicited by MMM (fuschia), in the right column. T cell responses were tested at 2 weeks following the 1^st inoculation, and then, at one week following each of the subsequent vaccine inoculations. Antibody responses were tested at 2 weeks following each inoculation. The memory response was tested at 13 weeks following the last inoculation (week 37). Data are medians with 25^th and 75^th quartiles indicated. Asterisks indicate responses that are significantly higher for one regimen than the other (*, p<0.05; **, p<0.01; ***, p<0.001). For example in panels (A) and (B), CD4 T cell responses were significantly higher in the DDMM regimen at weeks 17, 25 and 37; and at weeks 2 and 9 in the MMM regimen. The grey boxes represent the backgrounds for detection of specific responses.