Fig. 2.

Epifluorescence images of trophosome sections showing both symbionts located in bacteriocytes (b, bacteria) and host tissue (p, peritoneum; bv, blood vessel). All specimens were conserved in ethanol and embedded in LR White. FISH was conducted with 10% formamide and 3 h hybridisation time. Host-specific probes in Cy3 of (a1) Rp1752 Riftia pachyptila (# 1536-1), (b1) Tj202 Tevnia jerichonana (# 1536-2) and (c1) Oas154 Oasisia alvinae (# 1536-3). (a2, b2 and c2) symbiont specific Probe Rif445 in Fluos, targeting bacteria found in all 3 species. (a3, b3 and c3) DAPI staining of DNA. (a4, b4 and c4) overlay of host-specific probe (red), symbiont-specific probe (green) and DAPI (blue). Scale bar = 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)