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. Author manuscript; available in PMC: 2012 Sep 1.

Published in final edited form as: J Invest Dermatol. 2011 Nov 24;132(3 0 1):642–649. doi: 10.1038/jid.2011.377

Time-course of the relative expression of cytokines and iNOS in growing feathers collected from seven SLV chickens throughout vitiligo development. Relative expression was calculated by the delta delta Ct method, using a cDNA pool made from growing feather of 3 SL chickens that never developed vitiligo as the calibrator and chicken 28S as the endogenous control gene. The X-axis represent the time (weeks) of feather tip collection with respect to vitiligo onset (0); BV and CV represent data from feathers collected from the 7 SL chickens >2 weeks before vitiligo and >1 week after complete pigmentation loss, respectively; weeks −2 and −1 represent early vitiligo (EV) and weeks 0–4 correspond to active vitiligo (AV). (a) Expression pattern of IL-21, IL-10, and IFN-gamma. (b) Expression pattern of IL-1beta, IL-6, IL-8, IL-12beta, IL-15 and iNOS. Also see Table 2.

Time-course of the relative expression of cytokines and iNOS in growing feathers collected from seven SLV chickens throughout vitiligo development. Relative expression was calculated by the delta delta Ct method, using a cDNA pool made from growing feather of 3 SL chickens that never developed vitiligo as the calibrator and chicken 28S as the endogenous control gene. The X-axis represent the time (weeks) of feather tip collection with respect to vitiligo onset (0); BV and CV represent data from feathers collected from the 7 SL chickens >2 weeks before vitiligo and >1 week after complete pigmentation loss, respectively; weeks −2 and −1 represent early vitiligo (EV) and weeks 0–4 correspond to active vitiligo (AV). (a) Expression pattern of IL-21, IL-10, and IFN-gamma. (b) Expression pattern of IL-1beta, IL-6, IL-8, IL-12beta, IL-15 and iNOS. Also see Table 2.