Table 1.
RT-PCR primers sequences and conditions
| Gene name | RT-PCR primers (5′ to 3′) | RT-PCR conditions | ||||
|---|---|---|---|---|---|---|
| Forward | Reverse | Denaturing | Annealing | Elongating | Cycle no. | |
| CCR2 | GGAATCCTCCACACCCTGTTTC | ACCCAACTGAGACTTCTTGCTCCC | 94°C, 90 s | 55°C, 1 m | 72°C, 2 m | 38 |
| gp130 | TCAACTTGTGGAACCATGTGG | TCCAACTGACACAGCATGTTC | 94°C, 1 m | 66°C, 1 m | 72°C, 1 m | 35 |
| gp91phox | CTGAGCGAATTGTACGTGG | GCTTATCACAGCCACAAGC | 95°C, 30 s | 55°C, 45 s | 72°C, 45 s | 35 |
| ICAM-1 | GCCGCTCATTTACACCTATTA | TTCCTTTTCTTCTCTTGCTTG | 94°C, 90 s | 55°C, 1 m | 72°C, 2 m | 38 |
| IL-1r1 | CGAAAGACTATCAGTTTTTGGAAC | GTAACCTCGATGGTATCTTCCC | 94°C, 1 m | 62°C, 1 m | 72°C, 1 m | 35 |
| IL-1β | AAAGAAGAAGATGGAAAAGCGGTT | GGAACTGTGCAGACTCAAACTC | 95°C, 15 s | 59°C, 1 m | 72°C, 30 s | 35 |
| IL-6 | GACTGATGTTGTTGACAGCCACTGC | TAGCCACTCCTTCTGTGACTCTAACT | 94°C, 1 m | 62°C, 1 m | 72°C, 1 m | 35 |
| MCP-1 | TCTACAGAAGTGCTTGAGGTGGTTG | CCTGTTGTTCACAGTTGCTGCC | 94°C, 1 m | 60°C, 2 m | 72°C, 2 m | 35 |
| MIP-1α | CCCTTGCTGTTCTTCTCTGCAC | GCATTCAGTTCCAGCTCAGTGATG | 94°C, 1 m | 60°C, 2 m | 72°C, 2 m | 35 |
| MIP-1β | TCTGCGATTCAGTGCTGTCAGC | GATTTGCCTGCCTTTTTTGGTC | 94°C, 1 m | 60°C, 2 m | 72°C, 2 m | 35 |
| p22phox | TTGTTGCAGGAGTGCTATC | AATGGGAGTCCACTGCTAC | 95°C, 30 s | 55°C, 45 s | 72°C, 45 s | 35 |
| S16 | TCCGCTGCAGTCCGTTCAAGTCTT | GCCAAACTTCTTGGATTCGCAGCG | 95°C, 30 s | 58°C, 30 s | 72°C, 45 s | 28 |
| TNFr1 | GAACACCGTGTGTAACTGCC | ATTCCTTCACCCTCCACCTC | 94°C, 1 m | 62°C, 1 m | 72°C, 1 m | 35 |
| TNFα | ATGAGCACAGAAAGCATGATC | TACAGGCTTGTCACTCGAATT | 94°C, 90 s | 55°C, 1 m | 72°C, 2 m | 38 |