Table 3.
Summary of ANOVAs for brief-access licking
Source of variation | 3-way ANOVA |
Water-restricted 2-way ANOVA |
Tukey HSD (Ps < 0.05), water-restricted | Unrestricted 2-way ANOVA |
Tukey HSD (Ps < 0.05), unrestricted | ||||||
F | df | P | F | df | P | F | df | P | |||
Temperature constant (Figure 4A,B) | |||||||||||
Water-restriction status | 137.96 | 1,56 | <0.001 | ||||||||
Temperature | 6.38 | 3,56 | 0.001 | 1.86 | 3,28 | 0.17 | ns | 6.57 | 2,28 | 0 | 20, 30 > 40, 10 at ≥0.1 M |
Concentration | 481.75 | 3,168 | <0.001 | 117.74 | 3,84 | <0.001 | 0.2 > 0 M at 10, 20, 30, 40 | 531.57 | 3,84 | <0.001 | 0.2 > 0 M at 10, 20, 30, 40 |
Temperature × concentration | 2.36 | 9,168 | 0.02 | 0.55 | 9,84 | 0.83 | 8.68 | 9,84 | <0.001 | ||
Temperature × restriction | 0.32 | 3,56 | 0.81 | ||||||||
Concentration × restriction | 31.43 | 3,168 | <0.001 | ||||||||
Concentration × restriction × temperature | 4.19 | 9,168 | <0.001 | ||||||||
Temperature variable (Figure 4C,D) | |||||||||||
Water-restriction status | 136.8 | 1,56 | <0.001 | ||||||||
Temperature | 169.77 | 3,56 | <0.001 | 203.97 | 3,28 | <0.001 | 10 > 20 at 0.0 M; 10, 20, 30 > 40 at ≥0.0 M | 45.67 | 3,28 | <0.001 | 20, 30 > 10, 40 at ≥0.03 M |
Concentration | 217.1 | 3,168 | <0.001 | 44.42 | 3,84 | <0.001 | 0.2 >0 M at 20, 30, 40 | 210.13 | 3,84 | <0.001 | 0.2 > 0 M at 10, 20, 30, 40 |
Temperature × concentration | 15.49 | 9,168 | <0.001 | 5.9 | 9,84 | <0.001 | 18.98 | 9,84 | <0.001 | ||
Temperature × restriction | 6.57 | 3,56 | <0.001 | ||||||||
Concentration × restriction | 27.61 | 3,168 | <0.001 | ||||||||
Concentration × restriction × temperature | 8.65 | 9,168 | <0.001 |
df, degrees of freedom.