Fig. 2.

Fluorometric quantification of neutrophil extracellular trap (NET)-DNA from phorbol myristate acetate (PMA) (25 nM)-stimulated neutrophils after 3 h incubation in the absence or presence of (a) diphenylene iodonium (DPI) 25 µM and (b) superoxide dismutase (SOD) 95 units/ml. Data represent six independent experiments ± standard deviation (s.d.). Fluorometric quantification of NET-DNA (c) and reactive oxygen species (ROS) generation (d) from PMA (25 nM) or opsonized Staphylococcus aureus (300 per neutrophil)-stimulated neutrophils after 3 h incubation in the absence or presence of cytochalasin B (cytoB; 10 µg/ml). Data represent at least five independent experiments ± s.d. Luminol measures total ROS, isoluminol measures extracellular ROS and lucigenin measures extracellular superoxide. **P ≤ 0·01 by two-tailed paired t-test.