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. 2012 Feb;167(2):261–268. doi: 10.1111/j.1365-2249.2011.04518.x

Fig. 3.

Fig. 3

(a) Fluorometric quantification of neutrophil extracellular trap (NET)-DNA from phorbol myristate acetate (PMA) 25 nM-stimulated neutrophils after 3 h incubation in the absence or presence of catalase inhibitor, 3-aminotriazole (3-AT) 1 mM. Data represent six independent experiments ± standard deviation (s.d.) (b) Peak PMA 25 nM stimulated total reactive oxygen species (ROS) production measured by luminol. Data represent three independent experiments ± standard deviation (s.d.). *P ≤ 0·05 by two-tailed paired t-test. (c) Effect of 3-AT 1 mM on the activity of purified myeloperoxidase (MPO) 100 ng/ml compared to the specific MPO inhibitor, aminobenzoic acid hydrazide (4-ABAH) 1 mM (positive control). Experiment performed in triplicate ± s.d. Fluorometric quantification of NET-DNA from PMA 25 nM-stimulated neutrophils after 3 h incubation in the absence or presence of (d) glutathione precursor, NAC 10 mM or (e) 4-ABAH 0·1 mM. Data represent six independent experiments ± s.d. **P ≤ 0·01 by two-tailed paired t-test.