Fig. 5.

Time–course fluorescence photomicrographs (SYTOX staining) of neutrophils stimulated with (a) 0·75 mM hypochlorous acid (HOCl); (b) 1% Triton X-100 to induce cell lysis; (c) phosphate-buffered saline (PBS) negative control. PBS does not affect neutrophil viability to release nuclear DNA. Triton lyses cells immediately (0 min) to release DNA without neutrophil extracellular trap (NET) structure formation. HOCl stimulates NET-DNA release between 30 and 70 min post-stimulation. Bar represents 100 µm.