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. 2012 Feb;167(2):282–295. doi: 10.1111/j.1365-2249.2011.04496.x

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© 2012 The Authors. Clinical and Experimental Immunology © 2012 British Society for Immunology

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Fig. 4 — Peripheral blood mononuclear cells (PBMCs) were co-cultured with live or heat killed Lactobacillus acidophilus LAVRI-A1, Lb. rhamnosus GG, exopolysaccharides (EPS)-producing Streptococcus thermophilus St1275, Bifidobacterium longum BL536, B. lactis B94 or Escherichia coli TG1 in a ratio that does not induce apoptosis, lipopolysaccharide (LPS) or cells alone as control. The percentage of induced CD25⁺forkhead box protein 3 (FoxP3⁺) cells (a), and the induction of ROR-γt expressing T helper type 17 (Th17) cells (b), were assessed intracellularly by fluorescence activated cell sorter (FACS)Calibur after 96 h of co-culture. (b) Representative FACS plots for cultures described in (a); (d) Representative FACS plots for cultures described in (c). Data are expressed as means plus or minus standard error of the mean (s.e.m.) of three independent experiments.

Fig. 4 — Peripheral blood mononuclear cells (PBMCs) were co-cultured with live or heat killed Lactobacillus acidophilus LAVRI-A1, Lb. rhamnosus GG, exopolysaccharides (EPS)-producing Streptococcus thermophilus St1275, Bifidobacterium longum BL536, B. lactis B94 or Escherichia coli TG1 in a ratio that does not induce apoptosis, lipopolysaccharide (LPS) or cells alone as control. The percentage of induced CD25⁺forkhead box protein 3 (FoxP3⁺) cells (a), and the induction of ROR-γt expressing T helper type 17 (Th17) cells (b), were assessed intracellularly by fluorescence activated cell sorter (FACS)Calibur after 96 h of co-culture. (b) Representative FACS plots for cultures described in (a); (d) Representative FACS plots for cultures described in (c). Data are expressed as means plus or minus standard error of the mean (s.e.m.) of three independent experiments.