Table 1.
Development of DSS-colitis with association of Clostridium tyrobutyricum in conventional BALB/c and severe combined immunodeficiency (SCID) mice: clinical and histological gradings and detection of tumour necrosis factor (TNF)-α
| Body weight | Damage score | Mucin secretion | Length of colon | TNF-α | ||||
|---|---|---|---|---|---|---|---|---|
| Strain | Treatment | 2·5% DSS | Mortality/n | (g) | (0–4) | (4–0) | (cm) | (pg/10 mg tissue) |
| 1 BALB/c | C. tyrobutyricum (i.r.) | + | 0/5 | 18·8 ± 1·2 | 1·6 ± 0·5ΔΔΔ | 2·7 ± 0·4ΔΔΔ | 7·6 ± 0·5# | 27·9 ± 12·7## |
| 2 BALB/c | saline (i.r.) | + | 0/5 | 17·6 ± 1·9 | 3·9 ± 0·2ΔΔΔ | 0·1 ± 0·2ΔΔΔ | 6·9 ± 0·6# | 29·3 ± 15·3## |
| 3 BALB/c | saline (i.r.) | – | 0/5 | 19·6 ± 0·5 | 0 | 4 | 9·8 ± 0·7 | 7·8 ± 5·0 |
| 4 SCID | C. tyrobutyricum (i.r.) | + | 1/10 | 21·0 ± 2·1 | 0·5 ± 0·4ΔΔΔ | 3·5 ± 0·5ΔΔΔ | 10·0 ± 1·9* | 25·2 ± 5·2**,## |
| 5 SCID | saline (i.r.) | + | 2/10 | 20·0 ± 2·4 | 3·9 ± 0·1ΔΔΔ | 0·1 ± 0·2ΔΔΔ | 7·8 ± 0·8# | 79·3 ± 8·0## |
| 6 SCID | saline (i.r.) | – | 0/8 | 21·5 ± 1·2 | 0 | 4 | 10·9 ± 0·3 | 11·0 ± 2·2 |
Values are means ± standard deviation
*
P < 0·05
**
P < 0·01, significant difference of group 4 versus group 5
ΔΔΔ
P < 0·0001, significant difference of group 1 versus group 2 and group 4 versus group 5 of mice
#
P < 0·05
##
P < 0·01, significant difference of groups 1 and 2 versus control group 3 of BALB/c mice and group 4 and 5 versus control group 6 of SCID mice; i.r.: intrarectal administration. DSS: dextran sodium sulphate.