Figure 3. Relationships between network modules and behavioral traits.

(A) Top) Dendrogram of the subset of the area X network that includes the blue, dark green, orange, black, and salmon singing-related modules. ‘Leaves’ along ‘branches’ represent probes. The y-axis represents network distance as determined by 1 - TO, where values closer to 0 indicate greater similarity of probe expression profiles across samples. Color blocks below denote modules. Beneath, additional bands indicate positive (red) and negative (green) correlation (see scale bar in B). The top 2 bands show correlations to the number of motifs sung and the act of singing for probes in the dendrogram. The bottom 3 bands show the degree of correlation of these probes to the EGR1, FOXP2 and GAPDH probes with the most significant GS.motifs.X scores, respectively. ****passed Bonferroni for correlation to act of singing and number of motifs, and FDR for correlation to mean Wiener entropy; ***passed Bonferroni for correlation to act of singing and number of motifs, **passed Bonferroni for correlation to number of motifs and FDR for correlation to mean Wiener entropy; *passed FDR for correlation to number of motifs.

(B) Colors to the left represent the 21 proper modules in the network. For each module, the heatmap shows ME correlations to traits. Numbers in each cell report the correlation coefficients and Student asymptotic p-value (parentheses) for significant ME-trait relationships for the 5 singing-related modules as indicated by asterisks in (A). Scale bar, right, indicates the range of possible correlations from positive (red, 1) to negative (green, −1).