Figure 3. Partial silencing of RD29A-LUC in zdp mutant plants.
(A) Two-weeks old plants grown on MS plates were imaged for luminescence after cold treatment at 4°C for 2 days. (B) The expression levels of LUC and RD29A were determined by Real-time PCR. TUB8 was used as a control. Error bars represent s.d. (n=3). (C) Analysis of DNA methylation at transgene and endogenous RD29A promoter by CHOP-PCR (see experimental procedures for details). (D) Bisulfite sequencing analysis of promoter methylation status of endogenous RD29A promoter.