Figure 4.

Apoptotic response to a brief pulse of hid activity. (a) en-Gal4 >UAS-GFP; hs-hid wing disc showing high levels of anti-Caspase-3 (green) and TUNEL (red). GFP staining is shown in blue. A magnification is shown in (a′) to appreciate the concordance between anti-Caspase-3 and TUNEL staining. (b) en-Gal4>UAS-GFP UAS-dronc RNA-i; hs-hid showing a much reduced response in the posterior compartment. In the discs of genotype en-Gal4>UAS-GFP UAS-dp53 RNA-i; hs-hid (c) or en-Gal4>UAS-GFP UAS-puc; hs-hid (d), the apoptotic response in the posterior compartments is also much reduced. Note that TUNEL and anti-Caspase-3 staining are highly concordant in all cases