Figure 3.

TTRAP-positive nucleolar cavities do not accumulate proteins of ubiquitin proteasome system, Cajal bodies or splicing speckles. (a) Human SH-SY5Y cells were treated with 5 μM MG132 for 16 h. Double immunofluorescence was performed with anti-TTRAP (green) and anti-20S or anti-Ubiquitin (red) antibodies. (b) Double immunostaining of endogenous TTRAP (green) with components of Cajal bodies (FLASH and Coilin, as indicated) on SH-SY5Y cells treated as in (a). (c) SH-SY5Y cells were treated as in (a). Immunofluorescence with anti-TTRAP (green) and anti-SC35 (red), as marker of splicing speckles. Bars, 5 μm