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. 2011 Sep 16;19(3):488–500. doi: 10.1038/cdd.2011.118

Figure 5.

Figure 5

TTRAP accumulation in the nucleolus requires active rRNA transcription. (a) SH-SY5Y cells were treated with MG132 5 μM for 16 h, then the medium was replaced and the cells were treated with increasing concentrations of actinomycin D (ActD), as indicated. Control cells were either left untreated or treated with ActD alone. Indirect double immunofluorescence was performed with antibodies against TTRAP (green) and NPM (red). Bars, 5 μm. (b) Quantification of cells containing TTRAP-positive nucleolar granules. The percentage of cells with nucleolar TTRAP was estimated by counting at least 100 cells for each experiment, in three separate experiments. Significance was calculated with t-test (*P<0.05)