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. 2002 Sep;161(3):1007–1013. doi: 10.1016/S0002-9440(10)64261-7

Figure 2.

Figure 2.

MSP of p15 5′ CpG island. Primer sets for amplification are designated as unmethylated (U) and methylated (M). *, Molecular weight marker PhiX-HinfI; KG1A, positive control for M primer set and negative control for U primer set; HL60, positive control for U primer set and negative control for M primer set. Electrophoresis corresponds to the amplification of bisulfite-treated DNA from HL (lines 1 to 5). Line 6, no DNA control. HL in lines 1 and 5 showed methylation. Amplification with the U primers was observed in all of the primary lymphoma cases, which served as internal control of the DNA integrity after bisulfite treatment.