Figure 1.

RARE-dependent transcriptional activation depends on the 26S proteasome activity. (A) Cells were transfected with a RARE reporter and then treated with TTNPB (1 µM) in the presence or absence of MG132 (MG, 5 µM) for 16 h. Shown are fold inductions of the luciferase activities in relation to the untreated control. β-galactotsidase activity was used as an internal control. Error bars represent the standard deviations of three independent experiments. (B) Real-time RT-PC R analysis of the levels of endogenous RARβ mRNA following 16 h of ligand induction in the presence or absence of MG132, which are presented as fold variations compared to the untreated control. 18S rRNA was used as an internal control. Error bars represent the standard deviations of triplicates of one representative experiment.