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. 2011 Feb 1;6(2):202–211. doi: 10.4161/epi.6.2.13658

Figure 5.

Figure 5

The 26S proteasome activity is essential for ligand-dependent occupancy of RARβ2 promoter. (A) Schematic presentation of the relation of the DR5 RARE and TATA box at the promoter. (B) Real-time RT-PC R analysis of the levels of RARβ mRNA in cells treated with TTNPB (1 µM) in the presence or absence of MG132 (MG, 5 µM) for 1, 2 and 4 h. Quantification is presented as fold variations compared to the untreated control. 18S rRNA was used as an internal control. Error bars represent the standard deviations of triplicates from one representative experiment. (C) ChIP analysis of the occupancy of RARβ2 promoter by NCoR, RARα, RXRα, SRC-1, p300 and Pol II following 1, 2 and 4 h of ligand induction in the presence or absence of MG132. Lane 1 is the negative ChIP control. The input DNA was also analyzed in parallel. (D–F) Quantitative analysis of the association of NCoR, RARα and RXRα to the RARE is expressed as fold variations compared with the untreated controls after being normalized to the input controls. Error bars represent standard deviations of five independent experiments (**p < 0.01). (G) Following pretreatments with MG132 for 2 and 3 h and together with ligand for an additional hour, the occupancy of RARα and RXRα was examined by ChIP analysis.