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. 2011 Feb 1;6(2):202–211. doi: 10.4161/epi.6.2.13658

Figure 6.

Figure 6

The 26S proteasome activity is essential for ligand-dependent occupancy of R1, but not R2 region of Cyp26A1 promoter. (A) Schematic presentation of the R1 and R2 regions relative to the TATA box at Cyp26A1 promoter. (B) Real-time RT-PC R analysis of the levels of Cyp26A1 mRNA in cells treated with TTNPB (1 µM) in the presence or absence of MG132 (MG, 5 µM) for 1 and 4 h. Quantification is presented as fold variations compared to the untreated control. Error bars represent the standard deviations of the triplicates from one representative experiment. (C) ChIP analysis of the occupancy of Cyp26A1 regulatory region by RARα and RXRα following 1 and 4 h of ligand induction in the presence or absence of MG132. Lane 1 is the negative ChIP control. The input DNA was also analyzed in parallel.