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. 2011 Feb 1;6(2):202–211. doi: 10.4161/epi.6.2.13658

Figure 7.

Figure 7

Effects of proteasome inhibitor PS -341 on RAR-mediated transcriptional activation (A) Cells were treated with TTNPB (1 µM) in the presence or absence of PS-341 (10 or 20 nM) for 4 or 8 h and then subjected to western analysis of RARα and RXRα protein. The blots were then stripped and re-probed for γ-tubulin as an internal control. (B) Real-time RT-PCR analysis of the levels of RARβ and Cyp26A1 mRNA in cells treated with TTNPB and PS-341 (10 nM) for 1 and 4 h. Quantification is presented as fold variations compared to the untreated control. Error bars represent the standard deviations of the triplicates from one representative experiment. (C) ChIP analysis of the occupancy of RARβ2 and R1 and R2 region of Cyp26A1 by RARα in cells treated with TTNPB and PS-341. Lane 1 is the negative ChIP control. The input DNA was also analyzed in parallel. (D) The binding of RXRα was also analyzed by the ChIP assay.