Table I.
Panel of Isogenic DNA Repair-Deficient DT40 Cell Lines
| Name1 | Function | Reference |
|---|---|---|
| ATM2 | Checkpoint kinase, arrests cell cycle when chromosomal breaks are present. | Takao et al. (1999) |
| Brca2 | HR dependent DSB repair. | Hatanaka et al. (2005) |
| CtIP(S332A/−/−) | Eliminating covalently bound polypeptides from DSBs. | Nakamura et al. (2010) |
| CtIP(+/−/−) | Control clone for CtIP(S332A/−/−) | Nakamura et al. (2010) |
| FancC2 | Eliminating ICLs. | Kitao et al. (2006), Gurtan and D'Andrea (2006) |
| FancD2 | Eliminating ICLs. | Yamamoto et al. (2005) |
| Fen1 | BER, processing 5'flap in long patch and lagging strand DNA replication. | Matsuzaki et al. (2002) |
| Ku70 | Initial step for NHEJ dependent DSB repair. | Takata et al. (1998) |
| Nbs1/p70 | HR and initiation step for NHEJ. | Nakahara et al. (2009) |
| Pol-β2,3 | BER, repairs of basal damages caused by oxidative stress and environmental factors. | Horton et al. (2008) |
| Rad54 | HR dependent DSB repair. | Bezzubova et al. (1997) |
| Rad54/Ku702 | HR and NHEJ DSB repair | Takata et al. (1998) |
| Rev32 | TLS polymerase, involved in HR and also works epistatically with FancC in cross-linking tolerance. | Sonoda et al. (2003) |
| Rev3/FancC | TLS and ICLs repair. | Nojima et al. (2005) |
| Ubc132 | UBC13 is related to the initial step of HR and TLS. | Zhao et al. (2007) |
| XPA | Initial step of NER. | Okada et al. (2002) |
Abbreviations: BER = base excision repair, BRCA2 = breast cancer Type 2, CtIP = tumor suppressor protein, DSB = double strand break, FANCC = Fanconi anemia complementation group C, FANCD2 = Fanconi anemia complementation group D2, FEN1 = flap-endonuclease-1, HR = homologous DNA recombination, ICL = interstrand cross-linking, Ku70 = protein encoded by the XRCC6 gene that is required for non-homologous end joining, Nbs1/p70 = Nijmegen breakage syndrome, NER = nucleotide excision repair, NHEJ = non-homologous end joining, Pol-β = DNA polymerase-β, Pol-ζ = DNA polymerase-ζ, Rad54 = DNA-dependent ATPase, Rev3 = catalytic subunit of DNA polymerase ζ, TLS = translesion DNA synthesis, Ubc13 = Ubiquitin (Ub)-conjugating enzyme 13, XPA = xeroderma pigmentosum, complementation group A
DNA repair-deficient mutant clones used in qHTS and chromosome analysis.
Two isogenic clones were used.