Table II.

Known Rodent Carcinogenicity and Genotoxicity of Compounds Selected for Additional Investigation¹

Compound2	CASRN	Purity	Rodent Carcinogenicity	In Vitro Mammalian Cell Genotoxicity3	Ames Test Mutagenicity3	Known DNA repair pathway (Reference)
Actinomycin D	50-76-0	98%	Yes	positive	negative	Nbs1 (Porcedda et al., 2006)
Adriamycin	25316-40-9	98%	Yes	positive	positive	NER, HR (Spencer et al., 2008)
Alachlor	15972-60-8	99.2%	Yes	positive	-	Excision repair (Surrallés et al., 1995)
Benzotrichloride	98-07-7	98%	Yes	positive	negative4	none
Diglycidyl resorcinol ether	101-90-6	94%	Yes	positive	positive	none
Lovastatin	75330-75-5	≥98%	Yes	negative	negative	none
Melphalan	148-82-3	95%	Yes	positive	positive	Fanconi anemia pathway (Nojima et al., 2005)
Trans-1,4-Dichloro-2-butene	110-57-6	98%	Yes	positive	positive	none
Tris(2,3-epoxypropyl)isocyanurate	2451-62-9	99.5 %	Yes	positive	positive	none
2-Aminothiamine	96-50-4	>90%	Yes	positive	negative4	none

¹Abbreviations: CASRN = Chemical Abstracts Registry Service Number, ICL = interstrand cross-linking, Nbs1/p70 = Nijmegen breakage syndrome, NHEJ = non-homologous end joining.

²Diglycidyl resorcinol ether was purchased from Alpha Aesar; the other compounds were purchased from Sigma-Aldrich, except that lovastatin was purchased from Sigma-Aldrich and then again from ENZO Life Science.

³In vitro mammalian cell genotoxicity (e.g., tests for chromosomal aberrations, sister chromatid exchanges, or micronuclei in cultured Chinese hamster cells or mitogen-stimulated human lymphocytes, mutagenicity in mouse lymphoma cells) and Ames mutagenicity test results only in the absence of metabolic activation; test results were obtained from EPA ACToR and United States National Library of Medicine; TOXNET.

⁴Positive in the presence of metabolic activation.