The anti-allodynia effects of fibronectin are blocked by both para-chlorophenylalanine (p-CPA) and methysergide. Effects of p-CPA and methysergide on mechanical Allodynia were evaluated following treatment with vehicle, connecting segment-1 (CS-1), or fibronectin (FN), 2 or 5 weeks post-DCC. CS-1, fibronectin, or vehicle was administered immediately after DCC. p-CPA and methysergide were then administered at 2 or 5 weeks post-DCC, and mechanical allodynia was assessed (n=3 per group; *versus rats with no antagonist treatment).