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. Author manuscript; available in PMC: 2012 Feb 14.
Published in final edited form as: Traffic. 2010 Feb 27;11(6):827–842. doi: 10.1111/j.1600-0854.2010.01055.x

Figure 4. Expression of non-regulatable GRASP65 mutants inhibits mitotic Golgi disassembly when examined by EM.

Figure 4

A–E) Representative EM images of Golgi clusters (asterisks) in mitotic cells expressing indicated GRASP65 constructs. Mitotic cells were collected by shake-off of cells released from a double thymidine block and processed for EM. Bar, 0.5 μm. Note that the mitotic Golgi clusters were larger and more compact in cells expressing the mG mutant (C), the GRASP domain (D) and PDZ1 (E), with an increased number of remaining Golgi cisternal structures (arrowheads) in the clusters. F and G) Quantitation results of (A–E) to determine the number of mitotic clusters per mitotic cell profile (F) and the number of cisternae per mitotic cluster (G). Only cells with condensed chromosomes but no detectable nuclear envelope were counted. The number of cells (F) and clusters (G) quantified for each cell line was indicated and results expressed as the mean ± SEM. Statistical significance was assessed by comparison to the GFP cell line. ***p < 0.001.