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. Author manuscript; available in PMC: 2012 Feb 14.
Published in final edited form as: Traffic. 2010 Feb 27;11(6):827–842. doi: 10.1111/j.1600-0854.2010.01055.x

Figure 6. Expression of non-regulatable GRASP65 mutants does not significantly affect Golgi membrane distribution into the two daughter cells.

Figure 6

A–E) Confocal images of indicated HeLa cell lines in cytokinesis. Cells were induced by doxycycline for 72 h and fixed, stained for DNA and GM130. Asterisks indicate the two daughter cells. Scale bar, 20 μm. F) Relative distribution of the Golgi membranes in the two daughter cells using GM130 as a marker. The fluorescence intensity of GM130 in 10 pairs of daughter cells in each cell line was quantified using the NIH ImageJ software. The average (mean ± SEM) intensity in the daughter cells with relatively lower intensity compared with their pairs is shown in blue, and the average intensity in the cells with relatively higher GM130 signal is shown in red. The distribution of GM130 in the two daughter cells is statistically insignificant between all the cell lines (p > 0.05).