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. Author manuscript; available in PMC: 2012 Feb 14.
Published in final edited form as: Traffic. 2010 Feb 27;11(6):827–842. doi: 10.1111/j.1600-0854.2010.01055.x

Figure 7. Inhibition of mitotic Golgi fragmentation by expression of non-regulatable GRASP65 mutants delays mitotic entry and inhibits cell proliferation.

Figure 7

A) Inhibition of mitotic Golgi fragmentation delayed cell cycle progression. The percentage of cells in mitosis (mitotic index) at the indicated time-points of release from double thymidine block was determined for cells expressing GFP or GRASP65 constructs as indicated. Note that the cells expressing GFP or wild-type GRASP65 passed through mitosis in a cohort that peaks at 7 h. This peak was significantly delayed in the cells expressing the mG mutant, the GRASP domain or PDZ1 of GRASP65. B) BFA-induced Golgi disruption in cells expressing non-regulatable GRASP65 mutants rescued mitotic progression. As in (A), except that 5 μg/mL BFA was added to the tissue culture medium 4 h after the release from the double thymidine block. Note that the BFA treatment suppressed the mitotic delay in cells expressing the GRASP domain or PDZ1. C) Expression of non-regulatable mutants of GRASP65 reduced the cell growth rate. The cell number of each cell line was measured by staining the cells with crystal violet at indicated time-points and normalized with the cell number at the starting point. Note that the expression of the mG mutant, the GRASP domain or PDZ1 reduced the cell growth rate compared with expression of GFP or wild-type GRASP65.