Table 1.

Assay conditions for radioligand binding experiments.

Receptor/binding site	Receptor source	[Radioligand]	Non-specific binding in the presence of	Incubation time and temperature	Reference
Dopamine D1	Rat striatum	0.35 nM, [3H]SCH23390	3 μM, cis-flupenthixol	20 min, 37°C	Billard et al. (1984)
Dopamine D2	Rat striatum	0.15 nM, [3H]spiperone	3 μM, (+)butaclamol	15 min, 37°C	Fields et al. (1977)
Dopamine D3	Rat olfactory tubercles	0.70 nM, [3H]7-OHDPAT	1 μM, dopamine	60 min, 25°C	Lévesque et al. (1992)
Dopamine uptake site	Rat striatum	2.0 nM, [3H]WIN35,428	100 μM, cocaine	30 min, 4°C	Valchar and Hanbauer (1993)
Serotonin 5-HT1	Rat cortex	1.8 nM, [3H]-5HT	5 μM, 5-HT	20 min, 37°C	Bennet and Snyder (1976)
Serotonin 5-HT2	Rat prefrontal cortex	0.6 nM, [3H]ketanserin	3.6 μM, metergoline	20 min, 37°C	Leysen et al. (1981)
Serotonin 5-HT3	Rat entorhinal cortex	0.2 nM, [3H]GR65630	72 μM, quipazine	30 min, 37°C	Kilpatrick et al. (1987)
Serotonin uptake site	Rat forebrain	0.04 nM, [3H]paroxetine	100 μM, 5-HT	60 min, 22°C	Marcusson et al. (1988)
Benzodiazepines	Rat forebrain	1 nM, [3H]RO15-1788	10 μM, diazepam	90 min, melting ice	Chiu and Rosemberg (1983)
GABAA	Rat cortex and cerebellum	2.20 nM, [3H]muscimol	20 μM, GABA	30 min, 4°C	Yoneda and Kuriyama (1980)
GABAB	Rat cortex	10 nM, [3H]GABA	100 μM, (−)baclofen	10 min, 25°C	Enna and Snyder (1977), Herschel and Baldessarini (1979)
Chlorine channel	Rat whole brain	0.11 nM, [35S]TBPS	100 μM, picrotoxinin	120 min, 25°C	Corda et al. (1993)
AMPA	Rat cerebral cortex	8 nM, [3H]AMPA	1 mM, l-glutamate	60 min, 4°C	Murphy et al. (1987)
NMDA	Rat cerebral cortex	5 nM, [3H]CGP 39653	100 μM, l-glutamate	60 min, 4°C	Sills et al. (1991)
Kainate	Rat cerebral cortex	5 nM, [3H]kainic acid	1 mM, l-glutamate	60 min, 4°C	Monaghan and Cotman (1982)

GABA, γ-aminobutyric acid; TBPS, t-butylbicyclophosphorothionate; AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid; NMDA, N-methyl-d-aspartate.