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. 2012 Feb 1;26(3):282–293. doi: 10.1101/gad.180968.111

Figure 6.

Figure 6.

Genetic and nutritional rescue of rmn mutants. (A) Single-cell, homozygous rmn mutant embryo larvae were injected with wild-type zebrafish slc16a6a or human SLC16A6 cDNAs under the control of either bactin2 (broadly expressed) or fabp10a (liver-limited) promoter elements. Larvae were then stained with ORO on 6 dpf. (B) Whole-mount ORO staining of larvae fed from 5 to 12 dpf. The intestinal lumen contents were also stained with ORO, reflecting the lipid content of the larval diet. (C) Whole-mount ORO staining of larvae fed from 5 to 12 dpf and then removed from food. (D) In previously fed rmn mutants, hepatic steatosis appears after 3 d of fasting. (E) Survival curve for animals fed from 5 to 12 dpf and then removed from food (n = 50 larvae of each genotype). The median age at death was 20 ± 2 dpf for wild-type larvae and 18 ± 1.7 dpf for rmn mutant larvae (log rank test: χ2 = 62.3 on one degree of freedom; P = 2.9 × 10−16).