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. 2011 Aug;2(8):792–804. doi: 10.1177/1947601911431080

Figure 3.

Figure 3.

In BaF3 ErbB2/ErbB4 cells, anti-ErbB2 and anti-ErbB4 antibodies do not cross-react with the noncognate ErbB receptor. BaF3 ErbB2/ErbB4 cells were starved and stimulated with NRG2β. ErbB2 or ErbB4 was immunoprecipitated using ErbB2- and ErbB4-specific antibodies. (a) BaF3 cells that express various combinations of ErbB2 and ErbB4 constructs were generated by lentiviral and retroviral infections. (b) Immunoblotting was used to assess tyrosine phosphorylation (upper panel), ErbB4 expression (lower panel), and ErbB4 co-precipitation with ErbB2 receptor/antibody complex (lower panel). (c) Immunoblotting was used to assess tyrosine phosphorylation (upper panel), ErbB2 expression (lower panel), and ErbB2 co-precipitation with ErbB4 receptor/antibody complex (lower panel).