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. 2011 Aug;2(8):818–828. doi: 10.1177/1947601911431082

Figure 2.

Figure 2.

Knockdown of Pdcd4 up-regulates cyclin D1 expression. (A) The protein level of cyclin D1 in HT29-shLacZ and HT29-shPdcd4 cells was examined using Western blotting analysis. The ratio of cyclin D1/GAPDH in HT29-shLacZ cells is designated as 1.0. (B) The cyclin D1 mRNA levels in HT29-shLacZ and HT29-shPdcd4 cells were analyzed with real-time PCR. Three independent experiments were performed with triplicates for each sample every time. Results are expressed as mean ± standard deviation (n = 3). The ratio of cyclin D1/GAPDH in HT29-shLacZ cells is designated as 1. The asterisk indicates a significant difference by 1-way ANOVA (P < 0.001).