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Experimental range of the five numerical variables studied using rotatable CCD in terms of actual and coded factors.
| Factor | Name | Range of variables | ||||
|---|---|---|---|---|---|---|
| −α(−2.38) | Low (−1) | Mid (0) | High (+1) | +α (+2.38) | ||
| A | Inoculum Percentage (%) | 1.3 | 2 | 2.5 | 3 | 3.7 |
| B | Temperature (°C) | 23 | 30 | 35 | 40 | 47 |
| C | pH | 6.3 | 7 | 7.5 | 8 | 8.7 |
| D | Agitation (RPM) | 79 | 120 | 150 | 180 | 221 |
| E | Incubation time (h) | 55.5 | 72 | 84 | 96 | 112.5 |
