Table 4.

Plackett-Burman design for five variables with actual values along with the observed protease activity.

Sl. number	A	B	C	D	E	Protease activity (U)
1	3	40	7	180	96	257.23
2	2	40	8	120	96	166.03
3	3	30	8	180	72	176.52
4	2	40	7	180	96	275.47
5	2	30	8	120	96	134.25
6	2	30	7	180	72	218.98
7	3	30	7	120	96	147.30
8	3	40	7	120	72	150.14
9	3	40	8	120	72	122.58
10	2	40	8	180	72	219.67
11	3	30	8	180	96	201.53
12	2	30	7	120	72	136.41