Figure 18.
HPLC (High Pressure Liquid Chromatography) pattern of nucleosides of 5′ oligonucleotides of U1 RNA, U2 RNA, and U3 RNA [37]. The 5′ oligonucleotides of U1, U2, and U3 RNAs were obtained by digestion of T1 RNase and RNase A. The fragments were digested with snake venom phosphodiesterase and alkaline phosphatase. The nucleosides produced were separated by high pressure liquid chromatography (Varian Aerograph Liquid Chromatograph LCS-1000) at 55°C, 700–800 p.s.i. with 0.4 M ammonium formate (pH 3.5). Absorbance at 254 nm was recorded.