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Identification of m3 2,2,7G′ [16]. (a) Fluorogram of two-dimensional thin layer chromatography of [3H]-labeled nucleoside trialcohol derivative (N′) released from U1 RNA 5′ fragment. It was identified as m3 2,2,7G′ with standard. (b) Fluorogram of chromatographic separation of [3H]-labeled nucleoside trialcohol derivative on Whatman 3MM paper. The [3H]-labeled compound was identified as m3 2,2,7G′ with standard.
