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Two-dimensional chromatography in borate system [12]. The nucleoside mixture from U2 RNA 5′ oligonucleotide (RNase A product) was obtained by digestion with snake venom phosphodiesterase and alkaline phosphatase. The resulting nucleosides were separated with the borate system. Um and Am migrated through the butanol-boric acid while the m3 2,2,7G and C, which form borate complexes, were retained in the butanol-boric acid phase.
