Table 4. E. coli strains and plasmids used in this study.

Strains or plasmids	Relevant characteristics or genotype	Reference
Escherichia coli strains
DH5α	F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK − mK +), λ–	[67]
BL21 Rosetta 2	F− ompT hsdS B(rB − mB −) gal dcm (DE3) pLysSRARE2 (CamR)	Novagen
JM109	endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F′ traD36 proAB+ lacIq lacZΔM15] hsdR17(rK −mK +)	Promega
CY128	DH5α but Δggt ΔampC	[41]
Plasmids
pGEM®-T		Promega
pet28b(+)		Novagen
pBAD24	pMB1/M13 replicon, Ap+, AraC+, terminator (rrnB), promoter ParaBAD	[57]
pUC119	pMB1/M13 replicon, Ap+, lacZα,	[68]
pUOA14	pMB1/M13 replicon, pIP1445 replicon, lacZ′, Cm+, Ap+, Km+	[58]
pMRg1	pet28b(+), His6-Bgp1+	this study
pMRg2	pet28b(+), His6-Bgp2+	this study
pMRg3	pBAD24, Bgp1+	this study
pMRg5	pBAD24, Bgp2+	this study
pMRg9	pUC119, bgp2::cam +	this study