Table 1. MS analysis of ether glycerophospholipids from COS7 cells incubated with 50 µM polyene-lyso-ePE or -ePC for 2 or 1 h, respectively.
label | m/z | lipid species | rel. int. [%] | m/z | lipid species | rel. int. [%] |
lyso- | 638.4 | ePE(O-16:5/14:0) | 2 | 636.4 | ePE(P-16:5/14:0) | 2 |
ether- | 664.4 | ePE(O-16:5/16:1) | 23 | 662.4 | ePE(P-16:5/16:1) | 17 |
PE | 666.5 | ePE(O-16:5/16:0) | 8 | 664.4 | ePE(P-16:5/16:0) | 10 |
690.5 | ePE(O-16:5/18:2) | 21 | 688.4 | ePE(P-16:5/18:2) | 25 | |
692.5 | ePE(O-16:5/18:1) | 45 | 690.5 | ePE(P-16:5/18:1) | 45 | |
694.5 | ePE(O-16:5/18:0) | 2 | 692.5 | ePE(P-16:5/18:0) | 8 | |
714.5 | ePE(O-16:5/20:4) | 100 | 712.4 | ePE(P-16:5/20:4) | 100 | |
716.5 | ePE(O-16:5/20:3) | 8 | 714.5 | ePE(P-16:5/20:3) | 13 | |
lyso- | 740.5 | ePC(O-16:5/14:0) | 22 | |||
ether- | 766.5 | ePC(O-16:5/16:1) | 36 | |||
PC | 768.5 | ePC(O-16:5/16:0) | 55 | |||
792.5 | ePC(O-16:5/18:2) | 40 | ||||
794.5 | ePC(O-16:5/18:1) | 21 | ||||
796.5 | ePC(O-16:5/18:0) | 3 | ||||
814.5 | ePC(O-16:5/20:5) | 14 | ||||
816.5 | ePC(O-16:5/20:4) | 100 | ||||
818.5 | ePC(O-16:5/20:3) | 15 |
“O” indicates plasmanyl, “P” plasmenyl lipids.