Table 1. ANCOVA results of no-choice fertilization crosses.
species | source | df | type III SS | ms | F | P |
A. palmata eggs | ||||||
Log sperm (linear) | 1 | 1.33 | 1.33 | 18.83 | <0.0001 | |
Log sperm* log sperm (polynomial) | 1 | 0.04 | 0.04 | 0.51 | 0.479 | |
Cross | 1 | 3.84 | 3.84 | 54.56 | <0.0001 | |
Cross*log sperm | 1 | 0.42 | 0.42 | 0.59 | 0.440 | |
Cross*log sperm*log sperm | 1 | 0.06 | 0.06 | 0.81 | 0.37 | |
Error | 64 | 4.51 | 0.07 | |||
Total | 66 | 9.37 | ||||
A. cervicornis eggs | ||||||
Log sperm (linear) | 1 | 0.29 | 0.29 | 5.06 | 0.031 | |
Log sperm* log sperm (polynomial) | 1 | 0.27 | 0.27 | 4.67 | 0.037 | |
Cross | 1 | 0.08 | 0.08 | 1.41 | 0.243 | |
Cross*log sperm | 1 | 0.21 | 0.21 | 3.47 | 0.071 | |
Cross*log sperm*log sperm | 1 | 0.2 | 0.2 | 3.7 | 0.062 | |
Error | 36 | 2.08 | 0.06 | |||
Total | 39 | 2.76 |
ANCOVA was used to test differences in fertilization success as a function of cross type and sperm concentration. The dependent variable is the proportion of eggs fertilized (arcsine-transformed). The model consists of treatment group (conspecific vs. heterospecific cross) as the main effect, with sperm per milliliter (logistic transformation) as the covariate.