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. 2012 Feb 14;7(2):e30486. doi: 10.1371/journal.pone.0030486

Table 1. ANCOVA results of no-choice fertilization crosses.

species source df type III SS ms F P
A. palmata eggs
Log sperm (linear) 1 1.33 1.33 18.83 <0.0001
Log sperm* log sperm (polynomial) 1 0.04 0.04 0.51 0.479
Cross 1 3.84 3.84 54.56 <0.0001
Cross*log sperm 1 0.42 0.42 0.59 0.440
Cross*log sperm*log sperm 1 0.06 0.06 0.81 0.37
Error 64 4.51 0.07
Total 66 9.37
A. cervicornis eggs
Log sperm (linear) 1 0.29 0.29 5.06 0.031
Log sperm* log sperm (polynomial) 1 0.27 0.27 4.67 0.037
Cross 1 0.08 0.08 1.41 0.243
Cross*log sperm 1 0.21 0.21 3.47 0.071
Cross*log sperm*log sperm 1 0.2 0.2 3.7 0.062
Error 36 2.08 0.06
Total 39 2.76

ANCOVA was used to test differences in fertilization success as a function of cross type and sperm concentration. The dependent variable is the proportion of eggs fertilized (arcsine-transformed). The model consists of treatment group (conspecific vs. heterospecific cross) as the main effect, with sperm per milliliter (logistic transformation) as the covariate.