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. 2012 Feb 15;23(4):520–532. doi: 10.1091/mbc.E11-08-0704

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© 2012 Grubb et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Pdi1 physically interacts with ApoB29 and CPY*. Native immunoprecipitation reactions were performed using anti-HA resin or unconjugated Sepharose (Mock), using lysates from wild-type yeast strains expressing ApoB29 (A) or CPY* (B). In both cases, cell lysates were also examined that contained a vector control (–) in place of the ApoB29 or CPY* (+) expression vector. A total of 1% of the input for the precipitation was also examined (Lysate). After precipitation and SDS–PAGE, the indicated proteins were examined by immunoblot analysis.