FIGURE 4:

Migration of EGFR-mCherry expressing cells in a model of gliomatosis cerebri. (A) Migration of EGFR-mCherry–expressing cells was monitored in 300-μm-thick brain slices by time-lapse microscopy for 10 h. Individual cell tracks were transposed to a common origin to generate the resulting wind rose plots displayed in (A). Slices were treated with 0.1 ng/ml of EGF; EGF plus 100 ng/ml PDGF; EGF plus 10 μM Iressa; EGF plus 20 μM blebbistatin; EGF plus PDGF plus 10 μM Iressa; or EGF plus PDGF plus 20 μM blebbistatin. (B) MSD vs. time for the various treatments. The curves show the MSD the cells have traveled away from the common origin shown in the wind rose plots over time. The slope of the linear portion of the curves provides an estimate of the diffusion rate of the cells. Values of the diffusion constant (D) for each condition are summarized in Table 1. Code: EGF = 1; EGF + PDGF = 2; EGF + Iressa = 3; EGF + blebbistatin = 4; EGF + PDGF + Iressa = 5; EGF + PDGF + blebbistatin = 6. (C) Distribution of cell dispersal rates. The box plots show the distributions of dispersal rates of individual cells within a population. The middle red line shows the median dispersal rate; the top and bottom edges of the box are the 75th and 25th percentiles, respectively; the whiskers (black dashed lines) show the most extreme values not considered outliers; and the red +s are outliers. Notice that the vertical axis is log scale, so zero and negative values cannot be shown, and thus there are no bottom whiskers or outliers.