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. 2012 Feb 15;23(4):614–629. doi: 10.1091/mbc.E11-06-0584

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© 2012 Lacayo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Mobile ActA clusters with the actin comet tail and can generate persistently circling ellipsoidal beads. (A) The time-averaged projections of image sequences (actin and ActA) of a curving lipid-coated bead that migrates in the parallel orientation are shown. The average actin and ActA fluorescence on the surface of the bead were measured as depicted in the schematic on the right. Scale bar: 2 μm. (B) The average ActA and actin fluorescence intensities of the bead in (A) are plotted. The angle around the back surface of the bead is plotted on the x-axis (left side of the bead: 90°; back of the bead: 180°; right side of the bead: 270°). The distributions of both ActA and actin are asymmetrical, with increased localization on the left of the bead corresponding to the inside of the curve. The motility of this bead is characterized by an average angular velocity of 3.43°/s, average yaw angle of 9.67°, and average speed of 11.7 nm/s. (C) Ellipsoidal beads with lipid-coated surfaces and mobile ActA were often observed to migrate in tight persistent circles. This behavioral phenotype was extremely uncommon for uncoated beads. Scale bar: 2 μm. Time: min:s.