FIGURE 5:

The SibA protein is less stable in phg1A and sadA mutant cells than in wild-type cells. (A) Wild-type and mutant cells were incubated in the presence of cycloheximide for 0, 2, or 4 h and then lysed; the remaining SibA was detected by Western blotting after gel electrophoresis. To obtain equivalent signals at time zero, 10⁶ cells were loaded per lane for phg1A and sadA mutant cells and 10⁵ for wild-type cells. (B) The signals were quantified to determine the percentage of SibA remaining after 2 h of chase. The average and SEM of six independent experiments are indicated. *, significantly different from wild-type (p < 0.05 with Student's t test).