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. 2012 Feb 15;23(4):687–700. doi: 10.1091/mbc.E11-04-0380

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© 2012 Suzuki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Dynamics of the Sla1p patch and actin patch in EH domain mutants. (A) Average lifetimes of Sla1-GFP (left) and Abp1-mRFP (right) ± SD for indicated strains. Data were taken from 2-min movies with a 1-s frame interval. n = 50 patches for each strain. **p < 0.001. (B) Left, localizations of Sla1-GFP and Abp1-mRFP in live cells. Middle, kymograph representations of Sla1-GFP and Abp1-mRFP from the boxed area of strains as indicated. Right, quantification of fluorescence intensity (blue) and distance from the site of patch formation (red) as a function of time for patches of Sla1-GFP. Each curve represents data from one patch. Behavior of three independent patches was plotted for each strain. All movies were taken with a 1-s frame interval for both Sla1-GFP and Abp1-mRFP. The data in A were imaged as single-color movies using optimal GFP filter sets, whereas those in B were imaged as two-color simultaneous movies using narrow band-pass filter sets to completely separate GFP and mRFP fluorescence. Scale bars, 2 μm.