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. 2012 Feb 15;23(4):687–700. doi: 10.1091/mbc.E11-04-0380

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© 2012 Suzuki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Redundant function of EH mutants in endocytic patch formation. (A) The pan1^EH end3^EH and triple mutants were temperature sensitive for growth at 37°C. A dilution series of cells was plated on YPD medium and incubated at the indicated temperature. (B) Patch lifetimes of Sla1-GFP (top) and Abp1-mRFP (bottom) ± SD in the indicated strains. Data were obtained from 2-min movies taken with a 1-s frame interval. n = 50 patches for each strain. **p < 0.001. (C) Left, localization of Sla1-GFP and Abp1-mRFP from the strains indicated. Middle, kymograph representations of Sla1-GFP and Abp1-mRFP from the boxed area for the indicated times. Right, quantification of fluorescence intensity (blue) and distance from site of patch formation (red) as a function of time for patches of Sla1-GFP. Each curve represents data from one patch. Behavior of three independent patches was plotted for each strain. All movies were taken with a 1-s frame interval for both Sla1-GFP and Abp1-mRFP. Scale bars, 2 μm.