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. 2012 Feb 15;23(4):687–700. doi: 10.1091/mbc.E11-04-0380

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© 2012 Suzuki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Dynamics of Syp1p patch in EH domain mutants. (A, B) Distribution of Syp1p patch lifetimes in the indicated single (A) or double and triple EH domain mutants (B). Data were taken from ∼13-min movies with a 4-s frame interval. (C) Average lifetimes of Syp1-GFP ± SD for indicated strains. (D) Left, localization of Syp1-3GFP from the indicated strains. Yellow bars mark where the kymograph was generated. Right, kymograph representations of Syp1-3GFP from 5-min movies. Scale bars, 2 μm.