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. 2012 Feb 15;23(4):687–700. doi: 10.1091/mbc.E11-04-0380

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© 2012 Suzuki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Localization and temporal relationship of Syp1p and Sla1p patches in EH domain mutants. (A) Left, localization of Syp1-GFP and Sla1-mCherry (mCH) in live cells. Yellow bars mark where the kymograph was generated. Right, kymograph representations of Syp1-3GFP and Sla1-mCherry from 4- or 6-min movies. Arrowheads indicate examples of Syp1p patches that are joined by Sla1-mCherry. All movies were taken with a 3-s frame interval for both Syp1-GFP and Sla1-mCherry. Scale bars, 2 μm. (B) Temporal relationship of Syp1p, Sla1p, and Abp1p patches in EH domain mutants. Average time was obtained from at least 30 independent patches.