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. 2012 Feb 15;23(4):687–700. doi: 10.1091/mbc.E11-04-0380

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© 2012 Suzuki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — All Syp1p patches are eventually internalized in triple mutants. (A) Epifluorescence images of a single cell coexpressing Syp1-3GFP and Abp1-mRFP. Yellow bars mark where the kymograph was generated. Right, kymograph representations of Syp1-3GFP and Abp1-mRFP from 3-min movies. Arrowheads indicate examples of Syp1p patches that are joined by Abp1-mRFP. Scale bars, 2 μm. (B) Percentage of the Syp1-3GFP patches that were eventually joined by Abp1-mRFP. (C, D) Maximum-intensity projections of Z stacks of wild-type and triple-mutant cells labeled with Syp1-3GFP (C) or Abp1-mRFP (D). The Z series was acquired through the entire cell at 0.2-μm intervals. Dotted lines represent outline of cells. Quantification of Syp1-3GFP or actin patches/μm² ± SD in wild-type and triple mutant cells (n = 50 cells for each strain). Scale bars, 2 μm.