FIGURE 1:

The smc3-RQ allele promotes viability but the K113Q or QQ alleles do not. (A) Effect of smc3 acetyl-mimics on smc3-42 temperature sensitivity. Haploid VG3358 3B (smc3-42) alone (–) or with a second SMC3 allele, (WT) VG3377-1A, (K113Q) VG3423-1A, (QQ) VG3378-2A, or (RQ) VG3424-2A, grown at 23°C, plated at 10-fold serial dilutions on YPD, and incubated 3 d at 23, 30, 35.5, or 37°C. (B) Ability of smc3 acetyl-mimic alleles to support viability as the sole SMC3. Haploid VG3464-16C with pEU42 (SMC3 CEN URA3) and test plasmid bearing either no insert (–), SMC3 (WT), smc3K113Q (K113Q), or smc3K112R,K113Q (RQ) grown at 30°C, plated in fivefold serial dilutions on FOA or YPD, and incubated at 30°C.