FIGURE 4:

Sister chromatids segregate in smc3-42 mutants with or without acetyl mimics. Strains from Figure 2C smc3-42 mutant (smc3) or with a second SMC3 allele, WT, K113Q, or RQ, released from G1 at 35.5°C to allow one cell cycle before rearrest in G1. Chromosome IV monitored at a CEN4-proximal locus (TRP1). (A, B) Time course assessing cohesion loss. (A) Percentage of cells with two GFP signals. (B) DNA content. (C). Chromosome IV sister chromatid disjunction scored in large-budded cells. Dotted line marks the 50% disjunction expected for random segregation. For cohesion and nondisjunction assays, 100–400 cells were scored for each data point in each experiment.