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. 2012 Feb 15;23(4):729–739. doi: 10.1091/mbc.E11-08-0696

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© 2012 Guacci and Koshland. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Random segregation of sister chromatids in mcd1-1 cells released from nocodazole arrest. WT (VG3460-2A) and mcd1-1 mutant (VG3456-2C) cells arrested in mid M phase 35.5°C using nocodazole and then released from arrest (23°C). Chromosome IV monitored at a CEN4-proximal locus (TRP1). (A) Cohesion at mid-M-phase arrest. Percentage of cells with two GFP signals plotted for G1 and mid-M-phase cells. (B, C) Large-budded cells assayed 2 h after release from nocodazole. (B) Disjunction of chromosome IV sisters. Dotted line marks 50% disjunction expected for random segregation. (C) Micrographs showing chromosomal DNA (DAPI) and CEN-proximal locus of chromosome IV sisters (GFP). For cohesion and nondisjunction assays, 200–400 cells were scored for each data point.